In order to improve the ore-dressing index of a refractory gold ore from Gansu Province,based on the ore property,the flotation-tailings cyanide leaching process,raw ore roasting-cyanide leaching process,and biological oxidation-cyanide leaching process are compared.Results showed that with better conditions,the leaching rates of the biological oxidation-cyanide leaching process are 16.67 and 5.14 percentage points higher.Plus,the biological oxidation process requires less investment and low energy consumption and generates no pollutants;the biological oxidation-cyanide leaching process is more conducive to the effective development and utilization of gold and the leaching rate of 85.46 % can be reached.