In order for suitable and efficient exploitation and utilization of gold-bearing copper ore resources,experimental research on the flotation of a gold-bearing polymetallic copper ore with mixed oxide and sulfide content in Yunnan has been carried out.The results show that under optimal conditions,the closed circuit of the preferential copper and gold flotation-sulfur flotation process obtained the copper concentrate with gold grade 56.60 g/t,silver grade 652.25 g/t,copper grade 17.26 %,and gold recovery rate 63.63 %,silver recovery rate 61.17 % and copper recovery rate 74.38 %;sulfur concentrate with gold grade 2.35 g/t,silver grade 29.82 g/t,sulfur grade 47.33 %,and gold recovery rate 21.75 %,silver recovery rate 23.02 %,sulfur recovery rate 90.64 %;the total gold recovery rate is 85.38 %,total silver recovery rate is 84.19 %.The research results provide useful reference for the beneficiation of similar polymetallic ores.