The quality of filling bodies directly decides the quality of underground mining environment.Sampling from filling bodies is necessary for quality inspection of filling body.But conventional sampling of filling bodies will cause filling body damage.So the paper proposed sampling from filling bodies with diamond drilling machine and described the steps and testing of the method.The industrial application of this method shows that sampling from filling bodies with diamond drilling machine is not only simple and efficient,but also can realize sampling without damaging the sample.The method can be promoted in similar mines.