The biological oxidation heap leaching process where high-efficiency leaching strains are domesticated and cultivated is used to treat certain high magnesium and low-grade nickel sulfide ores from Jilin Province.The process conditions such as catalyst and column grain size,granulation,leaching pH and bacterial inoculation amount are studied.The results show that the leaching rates of nickel and copper are 72.22 % and 71.03 % respectively under the conditions:column grain size of -10 mm,leaching pH value of 2,bacterial inoculation amount of 30 %,biological oxidation heap leaching for 150 d,room temperature and addition of Ag＋ catalyst.The index is good.The study provides technical basis for the development and utilization of low-grade nickel sulfide ore resources.