Abstract: Bio-oxidation of refractory gold ore containing arsenic is a green environmental protection technology. Bio-oxidation of refractory gold ore containing arsenic has some disadvantages， such as a long reaction period and unsatisfactory leaching effect. Therefore， the addition of Ag^＋ catalyst to improve its oxidation efficiency is studied， and the influence of different Ag^＋ mass concentrations and leaching time on the bio-oxidation of refractory gold ore containing arsenic is discussed. The results showed that when 20 mg/L Ag^＋ is added， the highest arsenic leaching rate was 53.38 %. The redox potential continues to rise and finally tends to be stable. After Ag^＋ addition， the pH rises more than that without Ag^＋ addition， due to the fact that Ag^＋ addition generates more Fe^3＋ and provides more nutrients for bacteria. The ore samples in different oxidation stages are characterized by XRD， SEM-EDS and XPS， respectively. It is found that the addition of Ag^＋ can make the ore sample porous， increase arsenic leaching rate， accelerate bioleaching， shorten the bioleaching period， and improve oxidation efficiency.