Abstract: Based on the study of fluid inclusion， S isotope of pyrite， and C and O isotope of calcite in quartz and calcite in Daiwangshan Gold Deposit， the properties of ore-forming fluid， material source， and deposit genesis are discussed. The results show that fluid inclusions in quartz can be divided into 3 types： gas-liquid two-phase inclusions (type I)， CO₂-containing three-phase inclusions (type II)， and pure CO₂-phase inclusions (type III). The ore-forming fluid has the characteristics of medium temperature (171 ℃-280 ℃)， low salinity (5.4 %-11.7 %)， and low density (1.07-1.13 g/cm³). It is a H₂O-CO₂-CH₄ fluid system. The metallogenic depth is estimated to be 2.1-3.0 km， belonging to the shallow deposit. The δ³⁴S values range from -1.0 ‰ to -0.9 ‰， indicating that the sulfur in the ore-forming fluid originates from magmatic activity； the δ¹³C_V-PDB values range from -8.4 ‰ to -1.5 ‰， and the δ¹⁸O_V-SMOW values range from 6.3 ‰ to 19.7 ‰， suggesting that the sources of carbon and oxygen were multi-source. Based on the characteristics of ore-forming fluid and sources of ore-forming materials， it is considered that the genesis type of Daiwangshan Gold Deposit is a shallow orogenic type related to the closed orogenic paleo-Asian Ocean.