Abstract: Lithium concentrate is a natural mineral with a high grade of lithium, as well as abundant rubidium and cesium. Accurate determination of the content of these 3 elements is of great practical significance. Tetra-acid sample dissolution was used to dissolve lithium concentrate, and the content of lithium, rubidium, and cesium in lithium concentrate was determined by atomic absorption spectrometry in a dilute nitric acid medium. This method has high accuracy and good precision, with a spike recovery rate of 98.67 % - 99.67 %. The relative standard deviations of the measurement results were all less than 5 %, and they were consistent with the results obtained by the national standard method, making it suitable for industry promotion.