Abstract: To efficiently recover gold from sulfide and oxidized gold ore resources, a process mineralogy analysis was conducted on a mixed gold ore from Shandong Province, followed by orthogonal tests for flotation to investigate the influence of key factors on flotation performance and determine the optimal flotation process flow and indexes. The test results show that the degree of influence of each factor is as follows: copper sulfate dosage > grinding time > frother dosage > collector dosage. Copper sulfate dosage has a negative impact on flotation performance, while the frother provides limited improvement. In contrast, both collector dosage and grinding time exert positive effects when maintained within their respective optimal ranges. Under the conditions of grinding fineness of −0.074 mm accounting for 63.2 %, isoamyl xanthate of 80 g/t, aerofloat of 8 g/t, and 2^# oil of 20 g/t, a closed-circuit process of "one roughing, two cleaning, and three scavenging stages" is adopted, with middlings returned sequentially. Ultimately, a flotation concentrate with a gold grade of 41.53 g/t and a gold recovery of 94.23 % is achieved. The test results achieve good technical indicators for flotation.