Abstract: A high-sliming micro-disseminated gold ore with arsenic and carbon is characterized by complex mineral assemblages and fine-grained dissemination. Its treatment has long caused persistent technical issues including low gold recovery, excessive concentrate yield, and depressed concentrate grade. Poor mineral processing performance severely restricts the economic benefits of the enterprise, leading to significant difficulties in production and operation. To address these production bottlenecks, systematic flotation tests were conducted on this ore to optimize the separation process and reagent scheme. The research innovatively employed flash flotation to preferentially recover sulfide minerals with superior floatability, minimizing the loss of valuable minerals. Concurrently, high-performance composite depressants were applied to effectively disperse slime gangue minerals in finely ground slurry and suppress the entrainment and enrichment of gangue impurities. On the basis of maintaining stable gold recovery, the gold concentrate yield was substantially reduced, and the gold grade was remarkably enhanced. Optimum indicators were obtained with gold recovery of 82.43 %, concentrate yield of 13.63 %, and concentrate grade of 17.84 g/t. The optimized process conditions and innovative high-efficiency reagent scheme provide targeted solutions to on-site problems, deliver reliable technical support for industrial production, and significantly improve the mineral processing technical indices and the economic benefits of the enterprise.