Abstract: Bio-oxidation of refractory gold ore containing arsenic is a green environmental protection technology.Bio-oxidation of  refractory gold ores containing arsenic has some disadvantages,such as a long reaction period and unsatisfactory leaching effect.Therefore,the addition of Ag＋ catalyst to improve its oxidation efficiency is studied,and the influence of different Ag＋ mass concentrations and leaching time on the bio-oxidation of refractory gold ore containing arsenic is discussed.The results showed that when 20 mg/L Ag＋  is added,the highest arsenic leaching rate was 53.38 %.The redox potential continues to rise and finally tends to be stable.After Ag＋ addition,the pH rises more than that without Ag＋ addition,due to the fact that Ag＋  addition generates more Fe3＋ and provides more nutrients for bacteria.The ore samples in different oxidation stages are characterized by XRD,SEM-EDS and XPS,respectively.It is found that the addition of Ag＋ can make the ore sample porous,increase arsenic leaching rate,accelerate bioleaching,shorten the bioleaching period,and improve oxidation efficiency.