Defushenggou middle reaches gold deposit are situated within the East Kunlun Metallogenic Belt. To clarify the geological characteristics of the deposits and ascertain their prospecting potential, a comprehensive prospecting approach was adopted. This approach combined various methods, including geological reconnaissance surveys, stream sediment surveys, and induced polarization（IP）profile surveys. Through this integrated approach, a comprehensive analysis was conducted on the deposit’s geological, geochemical, and geophysical characteristics, followed by a summary of prospecting potential and a validation through the deployment of exploration projects. The ore bodies of Defushenggou middle reaches gold deposit are hosted within skarn and are controlled by near east west‐trending faults. The monzogranite and granodiorite of the Middle Triassic have provided the heat source and material source necessary for mineralization.The Jinshuikou Rock Group servesasthe primary ore‐hosting stratum,contributing partially to the material source for mineralization. Within the mining district, 3 alteration zones, 3 ore bodies, and 10 mineralized bodies have been delineated. Specifically, there is 1 silver ore body, 1 copper ore body,1 gold-silver polymetallic ore body, 6 copper mineralized bodies, 1 lead mineralized bodies, 2 gold-silver mineralized bodies, and 1 gold mineralized body. The stream sediment survey identified a total of 15 comprehensive anomalies（GA1-GA15）. Among them, comprehensive anomalies GA3, GA10, GA11, GA14, and GA15 demonstrated significant prospecting potential. The IP profile survey delineated 3 medium‐resistance and medium‐polarization anomalies（Ⅰ, Ⅱ, and Ⅲ）. Notably, Anomaly Ⅱ coincided precisely with the Ps1 and Ps20 structural fracture zones, indicating an ore‐induced anomaly with excellent prospecting potential. Based on the analysis of prospecting potential, exploration techniques such as trenching and drilling were employed to verify the deep extension of ore（mineralized）bodies in areas with prospecting potential and to investigate the metallogenic geological characteristics. This led to a breakthrough in prospecting, with the discovery of 4 gold mineralized bodies, named Kh7, Kh8, Kh14, and Kh16, respectively. The research findings can provide valuable references for fundamental geological research and prospecting breakthroughs for similar deposits within the region.