Abstract: A systematic separability experimental study was carried out for a low-sulfide gold-bearing ore in Guizhou,looking into the effects of grinding fineness,modifier and collector,and decided a reasonable separation process.The results show that under the optimal conditions,the closed-circuit flotation test can obtain the gold concentrate with productivity 3.45 %,gold grade 73.19 g/t and gold recovery rate 95.61 %,indicating good index.The research results provide an important technical reference for the separability evaluation and development of low-sulfide gold-bearing ore.