Abstract: The surrounding rock conditions of the second-step deteriorate due to the filtered water from the first-step stope in mechanized upward horizontal slicing and filling stoping.In light of that,the paper proposed and carried out experimental research on alternating-step upward horizontal slicing filling stoping method.The paper conducted the case study of V orebody of Xincheng Gold Mine,set up different step distance to the extraction process of the alternating-step upward horizontal slicing filling stoping,simulated and analyzed extraction process with Flac3D numerical simulation technology,and compared the displacement and stress of key points in the extraction process,in order to determine optimal step distance and ensure safe and efficient extraction of V ore body.Based on the results of stress displacement analysis under different working status,the paper determined the step distance between first-step and second-step stope to be 20.0 m,meaning that the optimal step distance is the first-step stope being 6 layers ahead of the second-step stope.The engineering application shows that the technical and economic index in experimental stope are: production capacity is 1 120 t/d,the mining loss rate is 4.6 % and ore dilution rate is 4.5 %.