Abstract: The quartz vein type gold ore from a gold mine in Yunnan contains 3.05 g/t gold,1.21 % sulfur,and 2.96 % iron.The main metal mineral is pyrite and gold is embedded in micro-fine grains.Based on the ore property,experimental research of mineral processing process was carried out.The results showed that when the grinding fineness was 80 %-0.074 mm,the regrinding fineness was 80 %-0.043 mm and the MA-3+ammonium dibutyl dithiophosphate was the combined collector for the stage grinding+grading+flotation flowsheet,the total gold concentrate with gold grade 47.60 g/t and gold recovery rate 88.64 % was obtained and the gold grade in total tailings is 0.37 g/t,achieving efficient utilization of gold resources.